Penerapan Ekstraksi Cair Tekanan Uap Tinggi Menggunakan Bahan Halal untuk Optimasi Antioksidan

Main Article Content

Akhmad Endang Zainal Hasan
Ike Yulia Wiendarlina
Witdiastuti Witdiastuti

Abstract

Soursop is a plant that is used as traditional medicine. Antioxidants are compounds that are able to delay, slow down, or inhibit oxidation reactions. This study aims to determine the effect of variations in extraction time and the comparison of variations in the amount of simplicia with aquadest solvent on the high pressure extraction method on antioxidant activity. The study made 13 treatments using the Response Surface Methodology (RSM) design with the fit of the Central Composite Design (CCD) model. The results showed that from the 13 treatments, the extraction time had an effect on the percent yield with the equation Y = -51.9692 + 24.2030 X1 + 1.2984 X2. The extraction time (X1) has a value greater than the ratio of simplicia powder to the volume of distilled water (X2) as well as the IC50 value as shown in the equation Y = 171,983 + 13,828 X1 – 4,110 X2. Extraction time (X1) has a greater value when compared to simplicia with aquadest solvent volume (X2). The results of the analysis of the percentage of extract yield and the optimal IC50 value using RSM analysis were thought to have an extraction time of 6.3 minutes and the ratio of simplicia powder to the volume of aquadest solvent was 1:61,2. The results of the antioxidant activity obtained that the optimal IC50 value was estimated at 4.3 minutes of extraction time and the ratio of simplicia powder to the volume of aquadest solvent was 1:78.2.

Article Details

How to Cite
Hasan, A. E. Z., Wiendarlina, I. Y., & Witdiastuti, W. (2022). Penerapan Ekstraksi Cair Tekanan Uap Tinggi Menggunakan Bahan Halal untuk Optimasi Antioksidan. JURNAL AGROINDUSTRI HALAL, 8(1), 116–127. https://doi.org/10.30997/jah.v8i1.5556
Section
Articles
Author Biographies

Ike Yulia Wiendarlina, Pakuan University

Pharmacy Program, Pakuan University

Witdiastuti Witdiastuti, Pakuan University

Pharmacy Program, Pakuan University

References

BPOM. (2014). Persyaratan Mutu Obat Tradisional. Badan Pengawas Obat Dan Makanan, 1–25.

Budiarti, A., Ulfah, M., & Oktania, F. A. (2016). Aktivitas Antioksidan Fraksi Kloroform Ekstrak Etanol Daun Sirsak (Annona muricata L.) dan Identifikasi Kandungan Senyawa Kimianya. Prosiding SNST Ke-5 Fakultas Teknik Universitas Wahid Hasyim Semarang, 2006, 7–12.

Cardoso, L. C., Serrano, C. M., Quintero, E. T., López, C. P., Antezana, R. M., & De La Ossa, E. J. M. (2013). High pressure extraction of antioxidants from Solanum stenotomun peel. Molecules, 18(3), 3137–3151. https://doi.org/10.3390/molecules18033137

Chaves, J. O., de Souza, M. C., da Silva, L. C., Lachos-Perez, D., Torres-Mayanga, P. C., Machado, A. P. da F., Forster-Carneiro, T., Vázquez-Espinosa, M., González-de-Peredo, A. V., Barbero, G. F., & Rostagno, M. A. (2020). Extraction of Flavonoids From Natural Sources Using Modern Techniques. Frontiers in Chemistry, 8. https://doi.org/10.3389/fchem.2020.507887

Chowdhury, S. S., Tareq, A. M., Tareq, S. M., Farhad, S., & Sayeed, M. A. (2021). Screening of antidiabetic and antioxidant potential along with phytochemicals of Annona genus: a review. Future Journal of Pharmaceutical Sciences, 7(1). https://doi.org/10.1186/s43094-021-00300-9

Cornelia, M., Natania, K., Cahyana, H., & Sutiyono, E. (2019). Encapsulation of Soursop (Annona muricata Linn.) Leaf Tea Extract Using Natural Mucilage. Reaktor, 19(1), 26–33. https://doi.org/10.14710/reaktor.19.1.26-33

Damasuri, A. R., Sholikhah, E. N., & Mustofa. (2020). Cytotoxicity of ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) on HeLa cell line. Indonesian Journal of Pharmacology and Therapy, 1(2), 54–59. https://doi.org/10.22146/ijpther.606

Faustin, S. D., Selestin, D., & Njintang, Y. N. (2017). Aqueous Extraction Optimization of the Antioxidant and Antihyperglycemic Components of Boscia Senegalensis Using Central Composite Design Methodology. Food Science & Nutrition, 3(1), 1–7. https://doi.org/10.24966/fsn-1076/100015

Gavamukulya, Y., Wamunyokoli, F., & El-Shemy, H. A. (2017). Annona muricata: Is the natural therapy to most disease conditions including cancer growing in our backyard? A systematic review of its research history and future prospects. Asian Pacific Journal of Tropical Medicine, 10(9), 835–848. https://doi.org/https://doi.org/10.1016/j.apjtm.2017.08.009

Góral, I., & Wojciechowski, K. (2020). Surface activity and foaming properties of saponin-rich plants extracts. Advances in Colloid and Interface Science, 279, 102145. https://doi.org/https://doi.org/10.1016/j.cis.2020.102145

Hannachi, H., Benmoussa, H., Saadaoui, E., Saanoun, I., Negri, N., & Elfalleh, W. (2019). Optimization of ultrasound and microwave-assisted extraction of phenolic compounds from olive leaves by response surface methodology. Research Journal of Biotechnology, 14(7), 28–37.

Hasan, A. E. Z., Mangunwidjaja, D., Sunarti, T. C., Suparno, O., & Setiyono, A. (2016). Antibreast cancer activity of nanopropolis Indonesia on induced mammary gland tumor by dmba in virgin sprague-dawley rats. Biotropia, 23(1), 35–41. https://doi.org/10.11598/btb.2016.23.1.473

Hasmila, I., Natsir, H., & Soekamto, N. H. (2019). Phytochemical analysis and antioxidant activity of soursop leaf extract (Annona muricata Linn.). Journal of Physics: Conference Series, 1341(3). https://doi.org/10.1088/1742-6596/1341/3/032027

Huang, H. W., Cheng, M. C., Chen, B. Y., & Wang, C. Y. (2019). Effects of high pressure extraction on the extraction yield, phenolic compounds, antioxidant and anti-tyrosinase activity of Djulis hull. Journal of Food Science and Technology, 56(9), 4016–4024. https://doi.org/10.1007/s13197-019-03870-y

Ince, A. E., Şahin, S., & Şümnü, S. G. (2013). Extraction of phenolic compounds from melissa using microwave and ultrasound. Turkish Journal of Agriculture and Forestry, 37(1), 69–75. https://doi.org/10.3906/tar-1201-1

Indriyanti, E., Purwaningsih, Y., & Wigati, D. (2017). Skrining Fitokimia dan Standarisasi. Cendekia Eksakta, 3(2), 20–25.

Khan, S. A., Aslam, R., & Makroo, H. A. (2019). High pressure extraction and its application in the extraction of bio-active compounds: A review. Journal of Food Process Engineering, 42(1), e12896. https://doi.org/https://doi.org/10.1111/jfpe.12896

Koocheki, A., Taherian, A. R., Razavi, S. M. A., & Bostan, A. (2009). Response surface methodology for optimization of extraction yield, viscosity, hue and emulsion stability of mucilage extracted from Lepidium perfoliatum seeds. Food Hydrocolloids, 23(8), 2369–2379. https://doi.org/https://doi.org/10.1016/j.foodhyd.2009.06.014

Kristanti, Y., Widarta, I. W. R., & Permana, I. D. G. M. (2019). Pengaruh Waktu Ekstraksi dan Konsentrasi Etanol Menggunakan Metode Microwave Assisted Extraction (MAE) Terhadap Aktivitas Antioksidan Ekstrak Rambut Jagung (Zea mays L.). Jurnal Ilmu Dan Teknologi Pangan (ITEPA), 8(1), 94. https://doi.org/10.24843/itepa.2019.v08.i01.p11

Kumar, S., & Pandey, A. K. (2013). Chemistry and Biological Activities of Flavonoids: An Overview. The Scientific World Journal, 2013, 162750. https://doi.org/10.1155/2013/162750

Liu, K. (2019). Effects of sample size, dry ashing temperature and duration on determination of ash content in algae and other biomass. Algal Research, 40, 101486. https://doi.org/https://doi.org/10.1016/j.algal.2019.101486

Montgomery, D. C. (2013). Design and Analysis of Experiments Eighth Edition. Arizona State University. In Copyright (Vol. 2009, Issue 2005).

Mustofa, S., Alfa, N., Wulan, A. J., Rakhmanisa, S., Biokimia, B., Molekular, B., Fisiologi, D., & Kedokteran, F. (2019). Pengaruh Pemberian Ekstrak Kulit Batang Bakau Minyak (Rhizophora apiculata) Etanol 95 % terhadap Arteri Koronaria Tikus Putih (Rattus novergicus) Jantan Galur Sprague dawley yang Dipaparkan Asap Rokok. Jurnal Kedokteran Universitas Lampung, 3(1), 28–33. http://juke.kedokteran.unila.ac.id/index.php/JK/article/view/2200

Mutakin, M., Fauziati, R., Fadhilah, F. N., Zuhrotun, A., Amalia, R., & Hadisaputri, Y. E. (2022). Pharmacological Activities of Soursop (Annona muricata Lin.). Molecules, 27(4), 1–17. https://doi.org/10.3390/molecules27041201

Orak, H. H., Bahrisefit, I. S., & Sabudak, T. (2019). Antioxidant activity of extracts of soursop (Annona muricata L.) leaves, fruit pulps, peels, and seeds. Polish Journal of Food and Nutrition Sciences, 69(4), 359–366. https://doi.org/10.31883/pjfns/112654

Putra, I. K. W., Putra, G. P., & Wrasiati, L. P. (2020). Pengaruh Perbandingan Bahan dengan Pelarut dan Waktu Maserasi terhadap Ekstrak Kulit Biji Kakao (Theobroma cacao L.) sebagai Sumber Antioksidan. Jurnal Rekayasa Dan Manajemen Agroindustri, 8(2), 167. https://doi.org/10.24843/jrma.2020.v08.i02.p02

Saifullah, M., Akanbi, T. O., McCullum, R., & Van Vuong, Q. (2022). Optimization of commercial microwave assisted-extraction conditions for recovery of phenolics from lemon-scented tee tree (Leptospermum petersonii) and comparison with other extraction techniques. Foods, 11(1). https://doi.org/10.3390/foods11010050

Santos-Sánchez, N. F., Salas-Coronado, R., Villanueva-Cañongo, C., & Hernández-Carlos, B. (2019). Antioxidant Compounds and Their Antioxidant Mechanism. In E. Shalaby (Ed.), Antioxidants. IntechOpen. https://doi.org/10.5772/intechopen.85270

Shahidi, F., & Zhong, Y. (2015). Measurement of antioxidant activity. Journal of Functional Foods, 18, 757–781. https://doi.org/https://doi.org/10.1016/j.jff.2015.01.047

Stéphane, F. F. Y., Jules, B. K. J., Batiha, G. E.-S., Ali, I., & Bruno, L. N. (2012). Extraction of Bioactive Compounds from Medicinal Plants and Herbs. In Intech (p. 13). http://dx.doi.org/10.1039/C7RA00172J%0A

Sumarni, W., Sudarmin, S., & Sumarti, S. S. (2019). The scientification of jamu: A study of Indonesian’s traditional medicine. Journal of Physics: Conference Series, 1321(3). https://doi.org/10.1088/1742-6596/1321/3/032057

Utami, Y. P., Umar, A. H., Syahruni, R., & Kadullah, I. (2017). Standardisasi Simplisia dan Ekstrak Etanol Daun Leilem ( Clerodendrum. Journal of Pharmaceutical and Medicinal Sciences, 2(1), 32–39.

Wahab, S. M. A., Jantan, I., Haque, M. A., & Arshad, L. (2018). Exploring the leaves of Annona muricata L. as a source of potential anti-inflammatory and anticancer agents. Frontiers in Pharmacology, 9(JUN), 1–20. https://doi.org/10.3389/fphar.2018.00661

Wang, W. H., Li, W. L., Chen, C. Y., Chang, M. Y., Huang, S. L., Shih, C. H., & Lin, Y. S. (2022). Antioxidant ability of Chenopodium formosanum extracted using an ethanol–ammonium sulfate two-phase system. Chemical and Biological Technologies in Agriculture, 9(1), 1–9. https://doi.org/10.1186/s40538-022-00283-6

Wu, S. J., Chen, Y. W., Wang, C. Y., & Shyu, Y. T. (2017). Anti-inflammatory properties of high pressure-assisted extracts of Grifola frondosa in lipopolysaccharide-activated RAW 264.7 macrophages. International Journal of Food Science & Technology, 52(3), 671–678. https://doi.org/https://doi.org/10.1111/ijfs.13320

Yudharini, G. A. K. F., Suryawan, & Wartini, N. M. (2016). Pengaruh perbandingan bahan dengan pelarut dan lama ekstraksi terhadap rendemen dan karakteristik ekstrak pewarna dari buah pandan (Pandanus tectorius). Jurnal Rekaya Dan Manajemen Argoindustri, 4(3), 36–46.

Yulianingtyas, A., & Kusmartono, B. (2016). Optimasi Volume Pelarut Dan Waktu Maserasi Pengambilan Flavonoid Daun Belimbing Wuluh (Averrhoa Bilimbi L.) Optimization of Solvent Volume and Maceration Time on Extraction of Flavonoids From Averrhoa Bilimbi Leaves. Jurnal Teknik Kimia, 10(2), 58–64.

Zambrano, M. V., Dutta, B., Mercer, D. G., MacLean, H. L., & Touchie, M. F. (2019). Assessment of moisture content measurement methods of dried food products in small-scale operations in developing countries: A review. Trends in Food Science and Technology, 88(December 2018), 484–496. https://doi.org/10.1016/j.tifs.2019.04.006

Zhang, Q. W., Lin, L. G., & Ye, W. C. (2018). Techniques for extraction and isolation of natural products: A comprehensive review. Chinese Medicine (United Kingdom), 13(1), 1–26. https://doi.org/10.1186/s13020-018-0177-x

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