THE QUALITY OF EMBRYOS RESULTED FROM IN VITRO FERTILIZATION BY USING FROZEN SEMEN THAWED IN DIFFERENT TEMPERATURES
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In vitro fertilization technology in cows is an effort done to utilize ovary waste from cows slughtered in abbatoir. This study was aimed at assessing the qualiy of embryos resulted from in vitro fertilization by using frozen semen thawed in different temperatures. In order to get qualty semen, standardized thawing method is required. It was expected from this study that an optimum thawing temperature for frozen semen was determined to obtain quality transferable embryos. Three treatments consisting of thawing with water 37°C for 30 second (T1), thawing with water 25°C for 30 second (T2), and thawing with water 10°C for 30 second (T3). Data were subjected to an an anlysis of variance (Anova) and a Duncan test. Results showed that oocytes fertilized with frozen semen thawed at 37°C and 10°C had higher fertilization rate and excellent-grade embryos (P<0.05) than did the ones fertilized with frozen semen thawed at 25°C. However, no different effect of thawing temperatures was found on transferable and degenerated embryos (P>0.05). It was concluded that embryos fertilized with Brahman frozen semen in thawed at 37°C had the highest number of embryos (49.66±2.88) and excellent-grade embryos (22.00±4.35).
Key words: Embryo quality, In vitro fertilization, frozen semen thawing, Brahman bull.
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Bavers MM, Dieleman S, Van den Hurk R, Radyar F. 1997. Rugalation and Modulation of Oocytes Maturation in The Bovine. Theriogenology. 47: 12-21.
Boediono A, Rusianto Y, Mohamad K, Djuwita I, Herliatien. 2000. Perkembangan Oosit Kambing Setelah Maturasi, Fertilisasi dan Kultur In Vitro. Media Veteriner. 7(4):11.
Chaiprasat S, Benjakul W, Chartchue A, Joemplang P, Punyapornwithaya V. 2006. Effect of Bull Semen Thawing Methods on Sperm Progressive Motility. Chiang Mai Veterinary Journal 4(1):25–29.
Darnel J, Lodish H, Baltimore D. 1990. Molecular Cell Biology.2 th ed.Sci. Am. Book.
Dhanju CK, Cheema RS, Kaur SP. 2001. Effects of Freezing On Protein and Profiles Of Sperm Membrane Extracts and Seminal Plasma of Buffalo Bulls. Journal of Department of Animal Breeding, College of Veterinary Sciences, Punjab Agricultural University, Ludhiana, India.
Einarsson S. 1992. Concluding Remarks. In: Influence of thawing method on motility, plasma membrane integrity and morphology of frozen stallion spermatozoa. Bor K, B Colenbrander, A Fazelli, J Pallevliet and L Malmgren (eds.) Theriogenology VI. 48th. 1997. Pp.531‐536.
Garner DL, Hafez ESE. 2000. Spermatozoa and Seminal Plasma. in Reproduction In Farm Animals. Edited by E. S. E. Hafez. 7th Edition. Lippincott Wiliams and Wilkins. Maryland. USA.
Goldman EE, Ellington JE, Farrel FB, Foote RH. 1991. Use Of Fresh And Frozen Thawed Bull Sperm Invitro. Theriogenology 35:204.
Gordon I. 2003. Laboratory Production of Cattle Embryos. 2nd ed. CABI Publishing, CAB International, Willingford, UK.
Grimes JF. 2008. Utilization of Embryo Transfer in Beef Cattle. ANR-17-8. http://ohioline.osu.edu/anr-fact/pdf/ANR_17_08.pdf [30 Mei 2015].
Lonergan P, Monaghan P, Rizos D, Boland M, Gordon I. 1994. Effect of follicle size on bovine oocyte quality and developmental competence following maturation, fertilization and culture in vitro. Molecular Reproduction and Development (37):48-53.
Nedambale TL, Du F, Yang X, Tian XC. 2006. Higher survival rate of vitrified and thawed in vitro produced blastocysts following culture in defined medium supplemented with βmercaptoethanol. Anim. Reprod. Sci. 93:61-75.
Nishizono H, Shioda M, Takeo T, Irie T, Nakagata N. 2004. Decrease of Fertilizing Ability of Mouse Spermatozoa After Freezing And Thawing Is Related To Cellular Injury. Biology of Reproduction 71:973–978.
Puchner AM. 2006. Novel follicular fluid factors influencing oocyte developmental potential in IVF: a review. Reproductive Bio Medicine Online 12(1):95-101.
Riandi A. 2001. Kajian Efektivitas Dosis Hormon Follicle Stimulating Hormone (FSH) dalam Metoda Superovulasi pada Ternak Sapi. [Skripsi]. Fakultas Kedokteran Hewan. Institut Pertanian Bogor.
Steel RGD, Torrie. 1995. Prinsip dan Prosedur Statistika Suatu Pendekatan Biometrik. Alih Bahasa B. Sumantri, Edition kedua, Cetatan 2. Penerbit PT. Gramedia Pustaka Utama, Jakarta.
Tammasia M, Nutinck F, May-Panloup P, Reynier P, Heyman Y, Charpigy G, Stojkovic M, Heindleder S, Renard JP, Chastant-Maillard S. 2004. In vitro embryo production efficiency in cattle, and its association with oocyte adenosine triphosphat content quantity mitochondrial DNA and mitochondriial DNA haplogroup. Biol. Reprod. 71:697-704.
Tanaka H, Herliantien, Herwiyanti E, Lubis OP, Buwono, Pujianto J. 2002. The Aftercare Technical Cooperation for The Strengthening of Artificial Insemination Center Project. Japan International Cooperation Agency. p.2
Tanghe S, Van Soom A, Nauwynck H, Coryn M, de Kruif A. 2002. Minireview: function of cumulus oophorrus during oosyte maturation, ovulation, and fertilization. In: Gene expression profil of cumulus cells derived from.
Toelihere MR. 1985. Fisiologi Reproduksi pada Ternak. CV. Angkasa. Bandung.
Widiastuti E. 2001. Kualitas Semen Beku Sapi FH Dengan Penambahan Antioksidan Vitamin C dan E. [Skripsi]. Fakultas Peternakan IPB. Bogor.
Zilli, Loredana, Roberto Sciavone, Vicenzo Zonno, Rocco Rossanao, Carlo Storelli, Sebastiano Viella. 2005. Effect of Cryopreservation on Sea Bass Sperm Protein. Journal Biolog of Reproduction. 72:1262-1267.
Zribi N, Chakroun NF, El Euch H, Gargouri J, Bahloul A, Keskes LA. 2010. Effects of cryopreservation on human sperm deoxyribonucleic acid integrity. Fertil Steril 93(1):159-166.