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Superovulation is a necessary technique to produce large number of embryos for embryo` transfer. Hormonal treatment is superovulation methode can be done by implant CIDR and injection of Follicle Stimulating Hormone (FSH). Experiment were carried out to observe the effectiveness of subcutaneous and intramuscular FSH injections on superovulation response in simmental cattle. All animal (n=10) were treated with intravaginal CIDR implant before FSH injection. Studies were devided into two experiment ie: P1 (400 mg FSH diluted in 4 ml sterile diluent) injected in five simmental cattle by single subcutan injection and P2 (400 mg FSH diluted in 20 ml sterile diluent) injected in five simmental cattle by twice daily intramuscular injection over 4 day in decreasing doses. The number of corposa luteal (CL), total embryos collected, and total transferable embryos were observed in this experiment. Data were analyzed by T-test method. The result showed that effectiveness of single subcutan FSH injection were significanthy different (P < 0,05) than intramuscular FSH injection superovulation with single subcutan FSH injetcion is easier than twice daily intramuscular injection in decreasy dose. In conclusion the average of CL (21,4 ± 3,6) and number of tranferable embryo (71,96 %) of the single subcutan FSH injection tended to be better than intramuscular FSH injection. Single subcutan FSH injection more efficient than intramuscular FSH injection. Single subcutan injection can decreasing sterss level in the cattle and be easier in handling the cattle during the experiment.
Keywords: Simmental cattle, FSH, superovulation, single subcutan injection, intramuscular injection.
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Amiruddin A, Siregar TN, Armansyah T, H Hamdan, Munandar A, Rifki M. 2013. Level steroid sapi aceh yang diinduksi dengan PMSG dan FSH. Jurnal Kedokteran Hewan. Vol.7 (2): 120 – 124.
Bo G, Hodey DK, Nasser LF, Mapleftoft RJ. 2010. Superovulation Response To a Single Subcutaneous Injection of Foltropin in Beef Cattle. Therogenologi 42 (6): 963-975.
Gordon IR. 2004. Reproductive Technologies in Farm Animals. CABI Publishing. London.
Hartantyo S. 1995. Calculation of percent progesterone in skim milk fraction when centrifugation temperature and butterfat of whole milk are known. Bull. FKH-UGM. Vol. XIV No. 2:1-6.
Hasler JF, Mapletof RJ. 2004. Embryo Transfer 101 With a Technical Slant. WCVM, University of Saskatchewan, Saskatoon, Saskatchewan Canada S7N 4, and Bioniche Animal Health USA, Inc (AB Technology),Pulman, WA 88163 USA
Kaiin EM, Tappa B. 2006. Induksi superovulasi dengan kombinasi CIDR, hormone FSH dan hCG induk sapi potong. Media Peternakan. 29(3):141-146.
McDonald. 2003. Veterinary Endocrinology and Reproduction. A Blackwell Publishing Company,Iowa.
Mapletoft RJ. 2006. Bovine Embryo Transfer. IVIS Reviews in veterinary Medicine, I.V.I.S. Western College of Veterinary Medicine, University of Saskatchewan, Canada.
Mapletoft RJ. 2012. The Evolution Of Improved and Simplified Superovulation Protocols in Cattle. Reproduction, fertility and Development. Western College of Veterinary Medicine, University of Saskatchewan, Canada.
Prasetyo D. 2012. Tingkat Superovulasi pada Beberapa Bangsa Nurhayat Sapi dengan Sumber Follicle Stimulating Hormone (FSH) yang Berbeda. [Skripsi]. Departemen Ilmu Produksi dan Teknologi Pertanian, Fakultas Peternakan, Institut Pertanian Bogor. Bogor.
Schallenberger E, Ulrich P, Most E, Fuchs S, Tenhumberg H. 1994. Induction of Superovulation In Cattle Comparing Single Subcutaneous and Repeated Epidural With Standard Intramuscular Administration of FSH. Therigenology 41(1) : 290.
Solihati N, Lestari D, Hidajati K, Setiawan R, Nurhayat LJ. 2006. Treatment Superovulasi Before Animal Slaughter. JIK. 6(2): 145-149.
Souza LB, Dupras R, Mills L, Chorfi Y, Price AC. 2013. Effect of synchronization of follicle wavve emergence with estradiol and progesteron and superstimulation with follicle-stimulating hormone on milk estrogen concentration in dairy cattle. The CJVR. 77 : 75-78.
Subhan FA, Handarini R, Siswanti SW. 2016. Perbedaan Waktu Penyuntikan Hormon FSH Terhadap Respon Superovulasi Sapi Angus. Jurnal peternakan Nusantara. Vol. 2 (1): 34 – 42.
Supriatna I. 2013. Transfer Embrio pada Ternak Sapi. Pusat Pengkajian Perencanaan dan Pengembangan Wilayah (P4W) Institut Pertanian Bogor. Bogor.
Vargas RB, Fukui Y, Miyamoto A, Terawaki Y. 1994. Estrus synchronization using CIDR in heifers. Journal of Reproduction and Development 40(1):59-64.