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July 18, 2019
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July 18, 2019
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July 18, 2019
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Abstract
Indonesia is a country that has a large biological diversity but has not been fully utilized for agriculture. The effectiveness of biological agents in controlling pests and plant diseases still needs further investigation, one of which is the use of entomopathogenic fungi as biopesticides. The purpose of this study was to determine the effect of storage on the viability and density of conidia in several entomopathogenic fungi; Beauveria bassiana, Metarhizium anisopliae, Paecilomyces. This study used 5 entomopathogenic fungi isolates, 2 Beauveria bassiana isolates (JBG and JBR), 2 Metarhizium anisopliae isolates (JBG and UST), 1 Paecilomyces. Biopesticide carrier material consisted of 6 carrier materials, namely soybean decoction waste, soy washing waste, Potato Dextrose Agar, tapioca waste, rice waste, rice washing waste and coconut water. Biopesticides have been made one year before and the viability and complexity of the conidia is re-examined one year later. From the observations of conidial density, the best was found in Beauveria bassiana JBG liquid biopesticide of 4.8 x 10 x conidia / ml from the initial density of 3.4 x 10⁸ / ml in the soybean decoction (RK) carrier material. The highest viability was found in JBG Metharizium anisopliae on soybean washing waste material by 14%. Key words: Conidia viability, conidial density, entomopathogenic fungi
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How to Cite
Triasih, U., agustina, dina, agustina, dina, dwiastuti, mutia erti, dwiastuti, mutia erti, wuryantini, susi, & wuryantini, susi. (2019). Test of Various Carrier Materials Against Viability and Conidia Density in Some Liquid Biopesticides of Entomopathogenic Fungi. JURNAL AGRONIDA, 5(1). https://doi.org/10.30997/jag.v5i1.1851
References
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Alves SB, Rossi LS, Lopes RB, Tamai MA, &. Pereira RM. 2002. Beauveria Bassiana Yeastphase on Agar Medium and Its Pathogenicity Againts Diatraea Saccharalis (Lepidoptera: Cerambidae) and Tetranychus Urticae (Acari: Tetranychidae). J. Invert. Pathol 81:70–77.
Aryo, Ketut, Lestari Wibowo, and Titik Nur Aeny. 2017. VIRULENSI BEBERAPA ISOLAT METARHIZIUM ANISOPLIAE TERHADAP ULAT GRAYAK ( Spodoptera Litura F ) Di LABORATORIUM. 5(2):96–101.
Batta, Y.2003. Production and Testing of Novel Formulations of the Entomopathogenic Fungus Metarhizium Anisopliae (Metschnikoff) Sorokin (Deuteromycotina: Hyphomycetes). Crop Protec 22(2):415–22.
Derakhshan, A., R.J. Rabindra, B. Ramanujam, and M. Rahimi. 2008. Evaluation of Different Media and Methods of Cultivation on the Production and Viability of Entomopathogenic Fungi Verticillium Lecanii (Zimm.) Viegas. Pak. J. of Biol. Sci 1–4.
Direktorat perlindungan tanaman pangan. 2014. Kementrian Pertanian Direktorat Jenderal Tanaman Pangan Direktorat Perlindungan Tanaman Pangan. Jakarta.
Djunaedy. 2009. Biopestisida Sebagai Pengendali Organisme Pengganggu Tanaman (OPT) Yang Ramah Lingkungan. Jurnal Fakultas Pertanian UNIJOYO.
Effendy TA, Septiadi R, Salim A, &. Mazid A. 2010. Jamur Entomopatogen Asal Tanah Lebak Di Sumatera Selatan Dan Potensinya Sebagai Agensia Hayati Walang Sangit (Leptocorisa Oratorius (F.). J. HPT Tropika 10(2):154–61.
Endri, N.2009. Evaluasi Kinerja Penanganan Limbah Cair Tahu Kita CV Kitagama. Universitas Gajah Mada. Yogyakarta.
Ferron P. 1981. Pest Control by Fungi Beauveria and Metarhizium. London: Academic Press.
Foxon, KM., S Pillay, T Lalbahadur, N Rodda, F. Holder and CA Buckley. 2004. The Anaerobic Baffled Reactor (ABR): An Appropriate Technology for on-Site Sanitation.
Gabriel, B. P. &. Riyatno. 1989. Metarhizium Anisopliae (Metch) Sor: Taksonomi, Patologi, Produksi Dan Aplikasinya. Jakarta: Direktorat Perlindungan Tanaman Perkebunan, Departemen Pertanian.
Harjaka, T. 2006. Isolasi Jamur Metarhizium Anisopliae Pada Hama Uret Perusak Akar Padi Gogo. Prosiding Seminar Hasil Penelitian Pertanian. Fakultas Pertanian UGM 200–205.
Hasyim A, Yasir H, &. Azwana. 2005. Seleksi Substrat Untuk Perbanyakan Beauveria Bassiana (Bals.) Vuill. Dan Infektivitasnya Terhadap Hama Penggerek Bonggol Pisang, Cosmopolites Sordidus Germar. J. Hort 15(2):116–23.
Herlinda.S. 2010. Spore Density and Viability of Entomopathogenic Fungal Isolates from Indonesia, and Their Virulence against Aphis Gossypii) Glover (Homoptera: Aphididae). J. Tropic. Life Scien. 21(1):13–21.
Magan, N. 2001. Physiological Approaches to Improving the Ecological Fitness of Fungal Biocontrol Agents. Di Dalam: Butt LM, Jackson CW, Magan N (Ed). Fungi as Biocotrol Agents: Progress, Problem and Potential. UK: Biddles Ltd, Guildford and King’s Lynn 239–52.
Meyling NV & Eilenberg J. 2007. Ecology of the Entomopathogenic Fungi Beauveria Bassiana and Metarhizium Anisopliae in Temperate Agroecosystems: Potential for Conservation Biological Control. J.Biol. Cont 43:145–55.
Munawaroh, S. dan A. Handayani. 2010. Ekstraksi Minyak Daun Jeruk Purut (Citrus Hystrix D.C.) Dengan Pelarut Etanol Dan N-Heksana. Jurnal Kompetensi Teknik. Jurnal Kompetensi Teknik 1(2):73–78.
Nuraida & Aisyah Lubis. 2016. Pengaruh Formulasi Dan Lama Penyimpanan Pada Viabilitas, Bioaktivitas Dan Persistensi Cendawan Metarhizium Anisopliae Terhadap Crocidolomia Pavonana Fabricius. J. HPT Tropika 16 No. 2(ISSN 1411-7525):196 – 202.
Prayogo Y, Tengkano W, &. Marwoto. 2005. Prospek Cendawan Entomopatogen Metarhizium Anisopliae Untuk Mengendalikan Ulat Grayak Spodoptera Litura Pada Kedelai. J. Litbang. Pertan. 24(1):19–26.
Prayogo Y. 2012. Virulensi Beberapa Isolat Cendawan Entomopatogen Beauveria Bassiana (Balsamo) Vuill. Untuk Mengendalikan Penggerek Ubi Jalar Cylas Formicarius. Prosiding Seminar Nasional Hasil Penelitian Tanaman Aneka Kacang Dan Umbi Tahun 2011. Pusat Penelitian Dan Pengembangan Tanaman Pangan, Badan Penelitian Dan Pengembangan Pertanian . Hal 738–54.
Prayogo Y. 2013. Toksisitas Cendawan Beauveria Bassiana Vuill. (Balsamo) Terhadap Telur Dan Larva Penggerek Ubi Jalar Cylas Formicarius (Coleoptera: Curculionidae). ). Prosiding Seminar Nasional Hasil Penelitian Tanaman Aneka Kacang Dan Umbi Tahun 2013. Pusat Penelitian Dan Pengembangan Tanaman Pangan, Badan Penelitian Dan Pengembangan Pertanian. Hal 669–81.
Rosalind, R. 2000a. The Effect of Certain Nutrients on Conidial Germination of Beauveria Bassiana and Paecilomyces Jumosoroseus. Agricultural Research Service, Tektran.
Rosalind, R. 2000b. The Effect of Certain Nutrients on Conidial Germination of Beauveria Bassiana and Paecilomyces Jumosoroseus. USDA: Agricultural Research Service, Tektran.
Samodra, H. And Y. Ibrahim. 2006. Effects of Dust Formulations of Three Entomopathogenic Fungal Isolates against Sitophilus Oryzae (Coleoptera: Curculionidae) in Rice Grain. J. Biosains 17(1):1–7.
Sheroze A, Rashid A, Shakir AS, &. Khan SM. 2003. Effect of Bio-Control Agents on Leaf Rustof Wheat and Influenceof Different Temperature and Humidity Levels on Their Colony Growth. Int. J. Agri. Biol 5(1):83–85.
Sri Sukamto. 1995. Pengaruh Kelembaban Relatif Terhadap Perkecambahan, Pertumbuhan Dan Sporulasi Beberapa Isolat Beauveria Bassiana. Pelita Perkebunan 11:64–74.
Taborsky. 1997. Small Scale Processing of Microbial Pesticides. Prague,Czechoslovakia: University of Agriculture.
Tanada, Y, and Kanaya. 1993. Insect Pathology. Academic Press, London
Y Srikandace, Y Hapsari, P Simanjuntak. 2007 Seleksi mikroba endofit Curcuma zedoaria dalam memproduksi senyawa kimia antimikrobia. Jurnal ilmu kefarmasian Indonesia. Jilid 5(2): 77-84
References
Alves SB, Rossi LS, Lopes RB, Tamai MA, &. Pereira RM. 2002. Beauveria Bassiana Yeastphase on Agar Medium and Its Pathogenicity Againts Diatraea Saccharalis (Lepidoptera: Cerambidae) and Tetranychus Urticae (Acari: Tetranychidae). J. Invert. Pathol 81:70–77.
Aryo, Ketut, Lestari Wibowo, and Titik Nur Aeny. 2017. VIRULENSI BEBERAPA ISOLAT METARHIZIUM ANISOPLIAE TERHADAP ULAT GRAYAK ( Spodoptera Litura F ) Di LABORATORIUM. 5(2):96–101.
Batta, Y.2003. Production and Testing of Novel Formulations of the Entomopathogenic Fungus Metarhizium Anisopliae (Metschnikoff) Sorokin (Deuteromycotina: Hyphomycetes). Crop Protec 22(2):415–22.
Derakhshan, A., R.J. Rabindra, B. Ramanujam, and M. Rahimi. 2008. Evaluation of Different Media and Methods of Cultivation on the Production and Viability of Entomopathogenic Fungi Verticillium Lecanii (Zimm.) Viegas. Pak. J. of Biol. Sci 1–4.
Direktorat perlindungan tanaman pangan. 2014. Kementrian Pertanian Direktorat Jenderal Tanaman Pangan Direktorat Perlindungan Tanaman Pangan. Jakarta.
Djunaedy. 2009. Biopestisida Sebagai Pengendali Organisme Pengganggu Tanaman (OPT) Yang Ramah Lingkungan. Jurnal Fakultas Pertanian UNIJOYO.
Effendy TA, Septiadi R, Salim A, &. Mazid A. 2010. Jamur Entomopatogen Asal Tanah Lebak Di Sumatera Selatan Dan Potensinya Sebagai Agensia Hayati Walang Sangit (Leptocorisa Oratorius (F.). J. HPT Tropika 10(2):154–61.
Endri, N.2009. Evaluasi Kinerja Penanganan Limbah Cair Tahu Kita CV Kitagama. Universitas Gajah Mada. Yogyakarta.
Ferron P. 1981. Pest Control by Fungi Beauveria and Metarhizium. London: Academic Press.
Foxon, KM., S Pillay, T Lalbahadur, N Rodda, F. Holder and CA Buckley. 2004. The Anaerobic Baffled Reactor (ABR): An Appropriate Technology for on-Site Sanitation.
Gabriel, B. P. &. Riyatno. 1989. Metarhizium Anisopliae (Metch) Sor: Taksonomi, Patologi, Produksi Dan Aplikasinya. Jakarta: Direktorat Perlindungan Tanaman Perkebunan, Departemen Pertanian.
Harjaka, T. 2006. Isolasi Jamur Metarhizium Anisopliae Pada Hama Uret Perusak Akar Padi Gogo. Prosiding Seminar Hasil Penelitian Pertanian. Fakultas Pertanian UGM 200–205.
Hasyim A, Yasir H, &. Azwana. 2005. Seleksi Substrat Untuk Perbanyakan Beauveria Bassiana (Bals.) Vuill. Dan Infektivitasnya Terhadap Hama Penggerek Bonggol Pisang, Cosmopolites Sordidus Germar. J. Hort 15(2):116–23.
Herlinda.S. 2010. Spore Density and Viability of Entomopathogenic Fungal Isolates from Indonesia, and Their Virulence against Aphis Gossypii) Glover (Homoptera: Aphididae). J. Tropic. Life Scien. 21(1):13–21.
Magan, N. 2001. Physiological Approaches to Improving the Ecological Fitness of Fungal Biocontrol Agents. Di Dalam: Butt LM, Jackson CW, Magan N (Ed). Fungi as Biocotrol Agents: Progress, Problem and Potential. UK: Biddles Ltd, Guildford and King’s Lynn 239–52.
Meyling NV & Eilenberg J. 2007. Ecology of the Entomopathogenic Fungi Beauveria Bassiana and Metarhizium Anisopliae in Temperate Agroecosystems: Potential for Conservation Biological Control. J.Biol. Cont 43:145–55.
Munawaroh, S. dan A. Handayani. 2010. Ekstraksi Minyak Daun Jeruk Purut (Citrus Hystrix D.C.) Dengan Pelarut Etanol Dan N-Heksana. Jurnal Kompetensi Teknik. Jurnal Kompetensi Teknik 1(2):73–78.
Nuraida & Aisyah Lubis. 2016. Pengaruh Formulasi Dan Lama Penyimpanan Pada Viabilitas, Bioaktivitas Dan Persistensi Cendawan Metarhizium Anisopliae Terhadap Crocidolomia Pavonana Fabricius. J. HPT Tropika 16 No. 2(ISSN 1411-7525):196 – 202.
Prayogo Y, Tengkano W, &. Marwoto. 2005. Prospek Cendawan Entomopatogen Metarhizium Anisopliae Untuk Mengendalikan Ulat Grayak Spodoptera Litura Pada Kedelai. J. Litbang. Pertan. 24(1):19–26.
Prayogo Y. 2012. Virulensi Beberapa Isolat Cendawan Entomopatogen Beauveria Bassiana (Balsamo) Vuill. Untuk Mengendalikan Penggerek Ubi Jalar Cylas Formicarius. Prosiding Seminar Nasional Hasil Penelitian Tanaman Aneka Kacang Dan Umbi Tahun 2011. Pusat Penelitian Dan Pengembangan Tanaman Pangan, Badan Penelitian Dan Pengembangan Pertanian . Hal 738–54.
Prayogo Y. 2013. Toksisitas Cendawan Beauveria Bassiana Vuill. (Balsamo) Terhadap Telur Dan Larva Penggerek Ubi Jalar Cylas Formicarius (Coleoptera: Curculionidae). ). Prosiding Seminar Nasional Hasil Penelitian Tanaman Aneka Kacang Dan Umbi Tahun 2013. Pusat Penelitian Dan Pengembangan Tanaman Pangan, Badan Penelitian Dan Pengembangan Pertanian. Hal 669–81.
Rosalind, R. 2000a. The Effect of Certain Nutrients on Conidial Germination of Beauveria Bassiana and Paecilomyces Jumosoroseus. Agricultural Research Service, Tektran.
Rosalind, R. 2000b. The Effect of Certain Nutrients on Conidial Germination of Beauveria Bassiana and Paecilomyces Jumosoroseus. USDA: Agricultural Research Service, Tektran.
Samodra, H. And Y. Ibrahim. 2006. Effects of Dust Formulations of Three Entomopathogenic Fungal Isolates against Sitophilus Oryzae (Coleoptera: Curculionidae) in Rice Grain. J. Biosains 17(1):1–7.
Sheroze A, Rashid A, Shakir AS, &. Khan SM. 2003. Effect of Bio-Control Agents on Leaf Rustof Wheat and Influenceof Different Temperature and Humidity Levels on Their Colony Growth. Int. J. Agri. Biol 5(1):83–85.
Sri Sukamto. 1995. Pengaruh Kelembaban Relatif Terhadap Perkecambahan, Pertumbuhan Dan Sporulasi Beberapa Isolat Beauveria Bassiana. Pelita Perkebunan 11:64–74.
Taborsky. 1997. Small Scale Processing of Microbial Pesticides. Prague,Czechoslovakia: University of Agriculture.
Tanada, Y, and Kanaya. 1993. Insect Pathology. Academic Press, London
Y Srikandace, Y Hapsari, P Simanjuntak. 2007 Seleksi mikroba endofit Curcuma zedoaria dalam memproduksi senyawa kimia antimikrobia. Jurnal ilmu kefarmasian Indonesia. Jilid 5(2): 77-84
Aryo, Ketut, Lestari Wibowo, and Titik Nur Aeny. 2017. VIRULENSI BEBERAPA ISOLAT METARHIZIUM ANISOPLIAE TERHADAP ULAT GRAYAK ( Spodoptera Litura F ) Di LABORATORIUM. 5(2):96–101.
Batta, Y.2003. Production and Testing of Novel Formulations of the Entomopathogenic Fungus Metarhizium Anisopliae (Metschnikoff) Sorokin (Deuteromycotina: Hyphomycetes). Crop Protec 22(2):415–22.
Derakhshan, A., R.J. Rabindra, B. Ramanujam, and M. Rahimi. 2008. Evaluation of Different Media and Methods of Cultivation on the Production and Viability of Entomopathogenic Fungi Verticillium Lecanii (Zimm.) Viegas. Pak. J. of Biol. Sci 1–4.
Direktorat perlindungan tanaman pangan. 2014. Kementrian Pertanian Direktorat Jenderal Tanaman Pangan Direktorat Perlindungan Tanaman Pangan. Jakarta.
Djunaedy. 2009. Biopestisida Sebagai Pengendali Organisme Pengganggu Tanaman (OPT) Yang Ramah Lingkungan. Jurnal Fakultas Pertanian UNIJOYO.
Effendy TA, Septiadi R, Salim A, &. Mazid A. 2010. Jamur Entomopatogen Asal Tanah Lebak Di Sumatera Selatan Dan Potensinya Sebagai Agensia Hayati Walang Sangit (Leptocorisa Oratorius (F.). J. HPT Tropika 10(2):154–61.
Endri, N.2009. Evaluasi Kinerja Penanganan Limbah Cair Tahu Kita CV Kitagama. Universitas Gajah Mada. Yogyakarta.
Ferron P. 1981. Pest Control by Fungi Beauveria and Metarhizium. London: Academic Press.
Foxon, KM., S Pillay, T Lalbahadur, N Rodda, F. Holder and CA Buckley. 2004. The Anaerobic Baffled Reactor (ABR): An Appropriate Technology for on-Site Sanitation.
Gabriel, B. P. &. Riyatno. 1989. Metarhizium Anisopliae (Metch) Sor: Taksonomi, Patologi, Produksi Dan Aplikasinya. Jakarta: Direktorat Perlindungan Tanaman Perkebunan, Departemen Pertanian.
Harjaka, T. 2006. Isolasi Jamur Metarhizium Anisopliae Pada Hama Uret Perusak Akar Padi Gogo. Prosiding Seminar Hasil Penelitian Pertanian. Fakultas Pertanian UGM 200–205.
Hasyim A, Yasir H, &. Azwana. 2005. Seleksi Substrat Untuk Perbanyakan Beauveria Bassiana (Bals.) Vuill. Dan Infektivitasnya Terhadap Hama Penggerek Bonggol Pisang, Cosmopolites Sordidus Germar. J. Hort 15(2):116–23.
Herlinda.S. 2010. Spore Density and Viability of Entomopathogenic Fungal Isolates from Indonesia, and Their Virulence against Aphis Gossypii) Glover (Homoptera: Aphididae). J. Tropic. Life Scien. 21(1):13–21.
Magan, N. 2001. Physiological Approaches to Improving the Ecological Fitness of Fungal Biocontrol Agents. Di Dalam: Butt LM, Jackson CW, Magan N (Ed). Fungi as Biocotrol Agents: Progress, Problem and Potential. UK: Biddles Ltd, Guildford and King’s Lynn 239–52.
Meyling NV & Eilenberg J. 2007. Ecology of the Entomopathogenic Fungi Beauveria Bassiana and Metarhizium Anisopliae in Temperate Agroecosystems: Potential for Conservation Biological Control. J.Biol. Cont 43:145–55.
Munawaroh, S. dan A. Handayani. 2010. Ekstraksi Minyak Daun Jeruk Purut (Citrus Hystrix D.C.) Dengan Pelarut Etanol Dan N-Heksana. Jurnal Kompetensi Teknik. Jurnal Kompetensi Teknik 1(2):73–78.
Nuraida & Aisyah Lubis. 2016. Pengaruh Formulasi Dan Lama Penyimpanan Pada Viabilitas, Bioaktivitas Dan Persistensi Cendawan Metarhizium Anisopliae Terhadap Crocidolomia Pavonana Fabricius. J. HPT Tropika 16 No. 2(ISSN 1411-7525):196 – 202.
Prayogo Y, Tengkano W, &. Marwoto. 2005. Prospek Cendawan Entomopatogen Metarhizium Anisopliae Untuk Mengendalikan Ulat Grayak Spodoptera Litura Pada Kedelai. J. Litbang. Pertan. 24(1):19–26.
Prayogo Y. 2012. Virulensi Beberapa Isolat Cendawan Entomopatogen Beauveria Bassiana (Balsamo) Vuill. Untuk Mengendalikan Penggerek Ubi Jalar Cylas Formicarius. Prosiding Seminar Nasional Hasil Penelitian Tanaman Aneka Kacang Dan Umbi Tahun 2011. Pusat Penelitian Dan Pengembangan Tanaman Pangan, Badan Penelitian Dan Pengembangan Pertanian . Hal 738–54.
Prayogo Y. 2013. Toksisitas Cendawan Beauveria Bassiana Vuill. (Balsamo) Terhadap Telur Dan Larva Penggerek Ubi Jalar Cylas Formicarius (Coleoptera: Curculionidae). ). Prosiding Seminar Nasional Hasil Penelitian Tanaman Aneka Kacang Dan Umbi Tahun 2013. Pusat Penelitian Dan Pengembangan Tanaman Pangan, Badan Penelitian Dan Pengembangan Pertanian. Hal 669–81.
Rosalind, R. 2000a. The Effect of Certain Nutrients on Conidial Germination of Beauveria Bassiana and Paecilomyces Jumosoroseus. Agricultural Research Service, Tektran.
Rosalind, R. 2000b. The Effect of Certain Nutrients on Conidial Germination of Beauveria Bassiana and Paecilomyces Jumosoroseus. USDA: Agricultural Research Service, Tektran.
Samodra, H. And Y. Ibrahim. 2006. Effects of Dust Formulations of Three Entomopathogenic Fungal Isolates against Sitophilus Oryzae (Coleoptera: Curculionidae) in Rice Grain. J. Biosains 17(1):1–7.
Sheroze A, Rashid A, Shakir AS, &. Khan SM. 2003. Effect of Bio-Control Agents on Leaf Rustof Wheat and Influenceof Different Temperature and Humidity Levels on Their Colony Growth. Int. J. Agri. Biol 5(1):83–85.
Sri Sukamto. 1995. Pengaruh Kelembaban Relatif Terhadap Perkecambahan, Pertumbuhan Dan Sporulasi Beberapa Isolat Beauveria Bassiana. Pelita Perkebunan 11:64–74.
Taborsky. 1997. Small Scale Processing of Microbial Pesticides. Prague,Czechoslovakia: University of Agriculture.
Tanada, Y, and Kanaya. 1993. Insect Pathology. Academic Press, London
Y Srikandace, Y Hapsari, P Simanjuntak. 2007 Seleksi mikroba endofit Curcuma zedoaria dalam memproduksi senyawa kimia antimikrobia. Jurnal ilmu kefarmasian Indonesia. Jilid 5(2): 77-84