PENGEMBANGAN PENANDA MOLEKULER BERDASARKAN SITUS SNP DAN INDEL GENOM KLOROPLAS KELAPA

Authors

  • Freta Kirana Balladona Institut Pertanian Bogor
  • Ismail Maskromo Balai Penelitian Tanaman Palma
  • Dewi Sukma Institut Pertanian Bogor
  • Sudarsono Sudarsono Institut Pertanian Bogor

DOI:

https://doi.org/10.30997/jag.v6i1.2548

Keywords:

dalam, genjah, SNAP, primer

Abstract

Saat ini informasi dasar mengenai silsilah, keragaman dan hubungan evolusi kekerabatan menggunakan marka molekuler pada kelapa di Indonesia masih kurang. Hal ini dibuktikan dengan belum banyak dilaporkan urutan sekuens genom kelapa Indonesia yang dapat dijadikan dasar dalam pembuatan marka molekuler tersebut. Salah satu genom tanaman yang dapat dimanfaatkan sebagai penanda adalah sekuens genom kloroplas (cpDNA). Genom kloroplas merupakan penanda yang efisien untuk mempelajari evolusi dan sejarah populasi tanaman melalui filogenetik karena bersifat sangat konservatif, diwariskan secara maternal, memiliki ukuran yang lebih kecil dibandingkan dengan genom inti. Tujuan dari penelitian ini adalah pengembangan primer berdasarkan genom kloroplas berbasis situ SNP dan indels. Berdasarkan sembilan sekuens genom kloroplas pada tanaman palma, telah berhasil didisain 10 primer berdasarkan situs SNP dan 5 primer berdasarkan situs insersi delesi. Hasil validasi primer tersebut menggunakan DNA kelapa Indonesia didapatkan hasil bahwa 10 primer SNP berhasil teramplifikasi sedangkan indels hanya 2 primer berbasis PCR.

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Published

2020-04-30

How to Cite

Balladona, F. K., Maskromo, I., Sukma, D., & Sudarsono, S. (2020). PENGEMBANGAN PENANDA MOLEKULER BERDASARKAN SITUS SNP DAN INDEL GENOM KLOROPLAS KELAPA. JURNAL AGRONIDA, 6(1), 1–13. https://doi.org/10.30997/jag.v6i1.2548

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